MTECH BIOTECHNOPLOGY(JNTU) MICROBIAL ENGINEERING SYLLABUS

M.Tech. (BIOTECHNOLOGY) - FIRST SEMESTER-COMMON SUBJECTS

                                       BT-101 MICROBIAL ENGINEERING 

UNIT I

Introduction to biotechnology and biochemical engineering, bioprocess techniques, biotechnology products. Raw materials used for Industrial fermentation and its processing. Chemical, physical and physiochemical treatment.

UNIT II

Material balance: Thermodynamic preliminaries, system and process, steady state and equilibrium, law of conservation of mass, types of material balance problem, material balances with recycle and bypass streams.

Energy balance: Basic energy concepts, intensive & extensive properties, Studies of enthalpy for reactive & non reactive processes. Heat of combustion, heat of reaction at Non standard conditions .Thermodynamics of microbial growth, energy balance equation for cell culture, unsteady state energy balance equations.

UNIT III

Medium optimization and Sterilization: Medium optimization techniques with special emphasize on statistical techniques, placket-Burman design, ANOVA, central; composite design, response surface methodology.

Sterilization: Media sterilization: kinetics of thermal death of cells & spores, design of batch and continuous thermal sterilization, coupling of Arhenious equation and cell death kinetics, sterilization of air and filter design. Radiation and chemical sterilization.

UNIT IV

Stoichiometry of bioreaction and energetic of microbial growth, ATP and red ox potential balance, Yield coefficients, Growth stoichiometry and elemental balances ,electron balances ,productivity and their correlation with the stoichiometry.

Unstructed model for microbial growth. The development of different microbial growth kinetics like Malthus, Pearl and read, Monod Model, Konark Model. The limitation of Monod model and development of other constitutive models. Multisubstrate model, inhibition models for substrate, Product and toxic substances, development of logistic equation. Maintenance and endogenous metabolism kinetics.

UNIT V

Kinetics based on molecular mechanism, Model for Plasmid Structured models - a few examples, Single cell model, Product formation expression and replication, model of gene expression, Segregated model.Models of plasmid stability. Thermal death kinetics of cells and spores. Engineering and social considerations for the production of r-DNA products;   Safety, Good Laboratory and manufacturing practices .Parameter estimation, Model validation and bioprocess optimization

TEXT BOOKS

1) Bailey JE, Ollis DF; Biochemical Engineering fundamentals Year of Publication 1986

2) Blanch HW and Clark DS: Biochemical Engineering Marcel Decker Year of Publication 1987

3)Introduction to Biochemical Engineering by D G Rao. Tata, Mc Graw Hill, New Delhi.

REFERENCES:

1)Biochemical Engineering Principles and functions by Syed Trnveer Ahmed Inamdar, PHI Learning Private limited.

2)Wiseman, A: Handbook of Enzyme Biotechnology, 3^rd Edition, Ellis Horwood Publication, Year of Publication 1999

3) Moser, A; Bioprocess technology, kinetics and reactors; Springer Verlag, Year of Publication1988

4) Schugerl K: Bellgart K H (Eds); Bioreaction Engineering, Modeling and control; Springer – verlog, berlin Year of Publication 2000